Bis-tris 丨 cas 6976-37-0

Bis-tris 丨 cas 6976-37-0yog siv dav davlamological tsisCompound, tseem ceeb rau nws muaj peev xwm tswj tau ruaj khov p cov kev cai nyob rau hauv biochemical thiab molecular biology kev sim. Nws yog ib feem ntawm tusTris-based Tsev Neeg, tsim los muab cov khoom muaj peev xwm ua tsis tau zoo hauvPh peb ntawm kwv yees li 5.8 txog 7.2, ua rau nws muaj txiaj ntsig tshwj xeeb rau kev nruab nrab rau cov teeb meem acidic me ntsis.

1. Biochemical thiab molecular biology buffering

Siv dav dav los tswjPhysiological PhHauv enzyme cov tshuaj tiv thaiv, protein purification, thiab nucleic acid ua haujlwm.

Qhov tsim nyog rau cov tsis xav tauNyob ruaj khov pH ntawm 6.5, xws li nyob rau hauv cov cia siab koom nrog ph-rhiab enzymes lossis cov protein.

Feem ntau siv nyob rau hauvElectrophoresis buffers, Xov Xwm Cell Kev Tshaj Tawm Xov Xwm, thiabchromatograph buffers.

2. Protein purification thiab crystallization

Bis-tris 丨 CAS 6976-37-0}} muajCov neeg muaj peev xwm ruaj khovThaum lub sij hawm protein purification txheej txheem xws liion-txauv cov chromatographythiabQhov loj me-cais tawm cov tshuaj tsw qab.

Siv nyob rau hauvCrystallization buffersTxhawm rau pab ruaj khov cov qauv protein thiab txhim kho cov siv lead ua kom zoo.

3. Kws ua tshuaj thiab cov qauv tshuaj ntsuam

Ua haujlwm hauvCov qauv tshuaj thiab cov tshuaj tiv thaivQhov twg tswj xyuas qhov tseeb pc qhov xwm txheej yog qhov tseem ceeb rau kev ruaj khov.

Siv nyob rau hauvimmunoassaysthiab lwm yam khoom siv kuaj mob uas yuav tsum muaj lub pH tswj cuav.

4. Enzyme cia thiab cov kev tshawb fawb biochemical

Txhawb nqaenzymatic kev ua siLos ntawm kev tswj hwm pH ib puag ncig zoo ib puag ncig.

Siv nyob rau hauv kev kawm txog enzymes nrog kev ua tau zoo tshaj plaws nyob rau hauv nruab nrab PH.

1. Kev ua tau zoo buffering

Buffers zoo nyob rau hauv lubPh ntau li 5.8 txog 7.2, ua rau nws zoo rau kev siv pH ze-nruab nrab.

PKA ≈ 6.5 ntawm 25 degree, kom ntseeg tau qhov muaj peev xwm ua tau zoo hauv qhov ntau no.

2. Tsawg Kub Siab Txaus Siab

Tsis zoo li qee qhov tsis txaus ntseeg, cov khoom pov thawj mob bis-trisTsawg tsawg hloov pauv ntawm kev sib txawv ntawm qhov kub thiab txias variations, muaj txiaj ntsig hauv kev sim ua ntawm kev hloov pauv hloov chaw kub.

3. Minimal Hlau Ion Binding

Qhiatsawg dua kev chelation ntawm cov hlau sib dhos hlau ions (piv txwv li, mg²⁺, Ca²⁺)Piv rau lwm tus tsis zoo li phosphate, yog li tsawg kawg kev cuam tshuam hauv cov khoom siv hlau-vam khom.

4. Lub zog dej siab thiab ruaj khov

Heev soluble hauv dej thiab kev siv tshuaj ruaj khov hla lub xov tooj dav thiab kub.

Tau tshaj cov roj ntsha molecules thiab reagents.

5. Kev sib raug zoo

Tau tshaj ntau cov roj ntsha molecules, suav nrog cov protein, nucleic acids, thiab cov dab tsi.

Tsis nqus tau qhov tseem ceeb hauv UV ntau yam, saib xyuas cov peev txheej spectrophotometric.

Bis-tris (CAS 6976-37-0)yog ib qho ntau yam thiab txhim khu kev qhalamological tsisdav siv nyob rau hauvBiochemistry, Molecular Biology, Tshuaj Kho Mob, thiabKev kuaj xyuas cov ntawv thovCov. Nws txoj kev pom ntawm lub siab ntev, qis ntawm cov tshuaj tiv thaiv hlau, thiab tshuaj xaiv kom zoo rau kev sim uas yuav tsum tau uaNyob ruaj khov pc tswj hwm nyob rau hauv nruab nrab acidic ib puag ncig.