1,3-bis [tris (hydroxymethyl) metylamino] propane are CAS 64431-96-5

1. Kev txheeb xyuas biological

Kab mob Bis-Tris yog ib qho dual-ntau zwitterionic buffer feem ntau siv hauv kev tshawb fawb txog roj ntsha, biochemical, thiab kev tshawb fawb molecular biology.

Cov ntawv thov tseem ceeb muaj xws li:

Electrophoresis (piv txwv li, SDS-nplooj, ib txwm muaj)

Enzyme kinetics thiab protein crystallization

Cell Kev cai thiab nyob rau hauv Verro ua yeeb yam

2. Molecular biology thiab biochemistry

Siv los tswj pH hauv:

Nucleic acid extraction thiab pcr kev

Tshuaj tiv thaiv kab mob antibody thiab conjugation workflows

Buffers rau DNA / RNA purification thiab hybridization

3. Electrophoresis thiab Gel Tshuab

Nyiam nyob rau hauv high-resolution gel tshuab vim nws ruaj khov tsis muaj peev xwm hla ob qho sib txawv phib pheeb suab.

4. Kws ntsuam xyuas thiab kuaj mob kuaj mob

Muaj nrog cov qauv tsim ua thiab cov khoom siv kuaj mob uas muaj cov khoom siv tsawg dua yog qhov tseem ceeb rau reagent kev ua tau zoo thiab ruaj khov.

1. Dual fuffering muaj peev xwm

Muaj ob qho kev siv tau muaj ntau dua (ze rau pH 7 thiab ph 9), muab kev yooj yim rau cov kev sim uas yuav tsum tau hloov PH lossis ntau kauj ruam.

2. Lub zog dej siab thiab ruaj khov

Yooj yim soluble thiab chemically ruaj khov, cia rau kev npaj ua kom yooj yim thiab ntev rau lub neej.

3. Minimal Hlau Ion Binding

Cov khoom pov thawj qis rau kev sib txawv rau cov neeg sib txawv xws li mg²⁺ thiab ca, kom tau kev sib xyaw nrog cov enzymes thiab cov tshuaj tiv thaiv.

4. Tsawg UV nqus

Haum rau UV-based Spectrophotometric ntsuas (piv txwv li, protein thiab nucleic acid animationification), yam tsis muaj kev cuam tshuam kho qhov muag.

5. BiCompatibility

Cov tshuaj lom tsis muaj tshuaj lom thiab muaj xyoob ntoo thiab cov khoom siv hauv xov tooj ntawm tes, tsim nyog rau cov ntaub ntawv ntawm tes nyob hauv, enzyme cia, thiab ntev-biochemical cov tshuaj tiv thaiv.

1,3-bis [tris (hydroxymethyl) metyllamino] propane (CAS 64431-96-5) yog ib qho kev txhawb nqa ntev hauv ob txoj cai. Nrog rau solubility zoo heev, ob lub peev xwm dual fuffering, thiab sib xyaw nrog cov roj ntsha, nws yog siv hauv cov roj ntsha, xyoob ntoo, electrophoresis, thiab kws tshuaj. Nws cov kev lom zem tsawg thiab kev cuam tshuam tsawg kawg nrog cov hlau ions ua nws txoj kev xaiv txhim khu kev qha rau ob qho kev tshawb fawb thiab kev siv cov ntawv thov.