Agarose 丨 cas 9012-36-6

Agarose 丨 cas 9012-36-6 yog cov polysaccharide tsim los ntawm cov chav tsev d-galactose thiab 3, {}} pob galactanose. Nws yog muab tau los ntawm agar, cov khoom noj kua roj ua tau muab rho tawm los ntawm qee cov marine algae. Agarose yog prized rau nws lub peev xwm los tsim thermoreversible gels nrog lub zog siab loj txawm nyob hauv cov kev ua kom muaj zog.
Agarose yog qhov dav nyob rau hauv biotechnology, molecular biology, chaw kuaj mob, tshwj xeeb rau cov txheej txheem tsim nyog rau DNA, RNA, thiab kev soj ntsuam.

1. GEL Electrophoresis (DNA \/ RNA Cais)
● agarose yog cov qauv kub rau kab rov tav gel electrophoresis.
● Siv los cais nucleic acids (DNA thiab RNA) raws li qhov loj me.
● Tso cai rau kev pom caj ces siv cov neeg sawv cev stateting (piv txwv li, kev coj phem bromide, SYBR ntsuab).
2. Protein purification (affinity chromatography)
● Cov kab hla agar
● Muaj ntau nyob rau hauv cov tshuaj tiv thaiv cov tshuaj tiv thaiv, enzymes, thiab fusion protein (piv txwv li, nws-tag lossis gst-tecificate).
3. Kev txhaj tshuaj tiv thaiv kab mob
● agarose tau siv nyob rau hauv radial imungoododiffusion thiab immunoelectrophoresis.
● Muab cov nruab nrab ruaj khov rau cov tshuaj tiv thaiv antigenity tiv thaiv kom pom thiab muaj nuj nqis.
4. Cell Kev cai thiab microbiology
● agarose tau siv los tsim cov semi-cov kev txhawb nqa rau microbial thiab cov tsiaj muaj kab lis kev cai.
● Melting point agarose tuaj yeem txhawb nqa cov hlwb los yog embryos.
5. Cov ntaub so ntswg thiab kev xa tshuaj
● Biocompadable thiab biodegradable agarose gels ua haujlwm ua scaffolds hauv cov ntaub so ntswg.
● Siv hauv kev tawm tshuaj tso tawm vim nws lub ntsej muag ntxig thiab hydrophilic xwm.
6. Kws ua tshuaj thiab cov tshuaj pleev ib ce
● Ua rau tus neeg sawv cev ntawm tus neeg sawv cev lossis cov tawv nqaij hauv chaw tsim khoom.
Siv rau hauv dermal fillers thiab tshuaj pleev ib ce vim nws tsis muaj tshuaj lom, hydrating cov khoom.

1. Lub zog siab ntawm cov kev ua kom muaj zog
● Cov ntawv muaj cov gels muaj zog (txawm nyob ntawm 0. 5-2% w \/ v) uas zoo tagnrho rau kev thov analalical.
Muab cov kev daws teeb meem zoo heev rau nucleic acid frium.
2. Thermoreversible gelation
● Melts ntawm ~ 90-95 degree thiab gels ntawm ~ 35-40 degree.
● Tso cai yooj yim, rov yaj, thiab tuav yam tsis muaj tshuaj hla kev hla.
3. Biocompible thiab tsis muaj tshuaj lom
● Inert thiab biodegradable, ua rau nws haum rau kev siv biomedical.
Tsis txhob cuam tshuam nrog kev siv roj ntsha lossis cov tshuaj tiv thaiv.
4. Txhais porosity
● Lub pege loj ntawm agarose gels yog tunable los ntawm kev kho kom haum.
● Zoo tagnrho rau qhov loj me-raws li biomolecules xws li DNA tawg lossis cov protein.
5. Tsawg tus keeb kwm yav dhau los hauv kev ntsuas cov txuj ci
● Pob tshab thiab qis autofluorescence, ua rau nws zoo tagnrho rau fluorescent staining.
● Kev sib cuam tshuam tsawg tsawg nrog kev txheeb xyuas cov txiaj ntsig huv thiab ua tau zoo.

Agarose 丨 cas 9012-36-6} Yog ib qho tseem ceeb, molifunctional biopolymer uas underpins ntau niaj hnub molecular biology thiab cov tswv yim biochemical. Nws lub peev xwm los tsim tsim nyog, thermoreversible gels nrog cov khoom siv hluav taws xob ua rau nws tsis tseem ceeb hauv electropraphy, tshuaj paug chratography, immunology, thiab cov ntaub so ntswg. Lub hauv paus chiv keeb ntuj, qis toxicity, thiab biocompatibility ntawm agarose ntxiv txhim kho nws tus nqi hla kev tshawb fawb, thiab kev lag luam.