Guanidine Thiocyanate 丨 Cas 593-84-0

Guanidine Thiocyanate 丨 cas 593-84-0 (gtc) yog tus neeg sawv cev chaotrotropic dav dav hauv molecular biology, biochemistry, thiab cov chaw soj ntsuam chaw soj ntsuam. Nws plays lub luag haujlwm tseem ceeb hauv nucleic acid rho tawm thiab protein denaturation. Ntawm no yog nws thawj cov ntawv thov thiab cov txiaj ntsig:

1. Nucleic acid extraction:

- RNA thiab DNA Kev sib cais: GTC yog siv nyob rau hauv cov lysis buffers rau Rabing RNA thiab DNA los ntawm cov hlwb thiab cov ntaub so ntswg. Nws pab cuam tshuam cov hlwb, denaturing cov protein, thiab inactivating nucleases (enzymes uas tuaj yeem txwv tsis taus lossis DNA).

- Viral RNA Extraction: Nws nquag siv nyob rau hauv extraction ntawm kev kuaj mob, xws li RT-PCTOn, xws li covid -19.

2. Protein Daus:

- Cov kev tshawb fawb tau protein: Guanidine Thiocyanate 丨 CAS {1}}}}}} yog ua haujlwm kom tsis txhob muaj cov tshuaj tiv thaiv tsis tau, nthuav tawm lawv mus rau hauv lawv cov ntaub ntawv. Qhov no muaj txiaj ntsig hauv kev kawm cov qauv protein thiab cov haujlwm.

- SDS-nplooj ntawv: GTC yog siv nyob rau hauv cov qauv npaj rau sodium dulecyl sulecyl sulecyl sulecyl sulecyl sulecyl sulfate-poly profailured thiab las voos sib cais.

3. Hybridier cia:

- Qaum teb thiab yav qab teb blotting: GTC tuaj yeem siv rau hauv hybridization buffers kom txhim kho qhov rhiab ntawm Northic acids hauv lub xeev denatured.

4. Cell lysis thiab purification:

- Cell liysis reagent: gtc yog ib feem ntawm cov lysis cell reagents siv nyob rau hauv huv nucleic acids lossis cov protein los ntawm ntau yam piv txwv.

- RAN stabilization: nws ruaj khov RNA los ntawm inactivating rneses thaum cov txheej txheem rho tawm.

5. Inactivation ntawm pathogens:

- Kev nyab xeeb hauv Decogostics: GTC yog qhov txiaj ntsig zoo hauv cov kab mob, cov kab mob, thiab lwm cov kab mob, tshwj xeeb tshaj yog thaum RNA rho tawm.

1. Zoo cell lysis:

- GTC tau txais txiaj ntsig ntawm cov tshuaj txhuam ntawm tes thiab cov protein thiab cov protein, tso cai rau kev nrawm thiab nthuav tawm cov nucleic acids.

2. NucTase inibition:

- Guanidine Thiocyanate 丨 cas 593-84-0} Cov txheej txheem ntawm nucleic actaction, uas tiv thaiv cov txheej txheem kev rho tawm, uas yog qhov tseem ceeb rau kev tau txais cov roj loj thiab DNA zoo.

3. Kev sib raug zoo nrog ntau txoj kev sib txuam:

- GTC tau sib haum nrog ntau lub nucleic acid extraction txoj kev, suav nrog cov ncej huv si symific, phenol-chloroform extraction, thiab sib nqus cov hau kev uas sib nqus.

4. Muaj txiaj ntsig zoo thiab purity:

- Kev siv GTC nyob rau hauv nucleic acid extraction cov txheej txheem feem ntau ua rau cov ntawv thov ntshiab thiab DNA, QPCr, thiab ntu.

5. Kab mob tsis sib haum xeeb:

- Nws lub peev xwm los ua kom tsis muaj kev nyab xeeb ua rau nws xaiv muaj kev nyab xeeb rau cov qauv kis tau, txo qis kev phom sij thiab kev nyab xeeb hauv chav kuaj.

6. Txhim kho cov rhiab hauv hybridization cia:

- GTC txhim kho cov rhiab heev ntawm hybridization cia, ua rau kom muaj ntau yam kev thov, suav nrog cov khoom siv tsom xam thiab kuaj mob.

Cov kev ceev faj:

- Toxicity: GTC yog tshuaj lom thiab muaj peev xwm ua rau muaj kev phom sij yog nqus tau, noj, lossis los rau hauv kev sib cuag nrog daim tawv nqaij. Nws yuav tsum tau daws nrog kev saib xyuas, siv cov khoom tiv thaiv tsim nyog.

- Corrosive: GTC yog corrosive thiab tuaj yeem ua rau kev puas tsuaj rau cov ntaub ntawv xws li cov hlau thiab qee qhov plastics, nrog rau kev kub ntxhov thiab lub qhov muag.

- Kev nyab xeeb ib puag ncig: nws yuav tsum tau muab pov tseg kom zoo, raws li nws tuaj yeem tsim kev puas tsuaj rau ib puag ncig yog tias tsis tswj kom raug.

Guanidine Thiocyanate 丨 Cas 593-84-0 yog qhov muaj zog thiab ntau yam nucleic acids thiab inactivates cov peev xwm paug.